88: SULFOLOBUS MEDIUM
Modifications: Reduce the amount of yeast extract to 0.20 g/l and supplement the medium with 5.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and add aseptically to the autoclaved medium.
Growth observed:
Growth conditions: 65 °C